Results of intensive phase tretament of tuberculosis treatment according to CYP2E1 genotype

The polymorphism of the gene of cytochrome P-450 2E1 (CYP2E1) that participate in metabolism of antituberculosis agent isoniazid may influence on effectiveness of tuberculosis (TB) treatment. Aim of research: to detect the peculiarities of pulmonary TB course and outcome after in -patient treatment according to CYP2E1 genotype of the patients with primary TB. Materials and methods: analysis of medical cards from 86 patients with primary pulmonary tuberculosis at the end of hospital treatment in Odessa regional antituberculosal dispensary was conducted in 2012 year with consideration of CYP2E1 genotype. Results: the obtained data has proved that at the beginning of treatment there was no significant difference between TB patients concerning the signs of destruction, infiltration and disintegration according to CYP2E1 polymorphism, however the patients with CD, DD genotype more frequently had sings of dissemination and destruction in pulmonary tissues than the patients with CC genotype. At the end of in-patient treatment the patients with CD, DD genotype more often exhibited signs of infiltration of pulmonary tissues and longer remained smear-positive according to cultural method, than the patients with CC genotype. Despite of CYP2E1 genotype around 25% of tuberculosis patients had multidrug resistant strains of M.tuberculosis in the end of in-patient treatment. It will be important to check in coming researches an influence of CYP2E1 polymorphism in TB-patients on toxicity of antituberculosis therapy

Disagreement between capture probabilities extracted from capture and quasi-elastic backscattering excitation functions

Experimental quasi-elastic backscattering and capture (fusion) excitation functions are usually used to extract the s-wave capture probabilities for the heavy-ion reactions. We investigated the $^{16}$O+$^{120}$Sn,$^{144}$Sm,$^{208}$Pb systems at energies near and below the corresponding Coulomb barriers and concluded that the probabilities extracted from quasi-elastic data are much larger than the ones extracted from fusion excitation functions at sub and deep-sub barrier energies. This seems to be a reasonable explanation for the known disagreement observed in literature for the nuclear potential diffuseness derived from both methods.Comment: 9 pages, 3 figure

Outcomes of tuberculosis treatment depending on CYP2C19 genotype

As proven earlier, in patients with tuberculosis (TB) the concentration of antituberculosis drugs like isoniazid and rifampicin may differ depending on polymorphism of CYP2C19. The aim of the research was to determine the peculiarities of the pulmonary TB course and its outcome after the in-patient treatment depending on CYP2C19 genotype of the patients. The analysis of medical cards from 83 patients with the primary pulmonary tuberculosis at the end of the in-patient treatment in Odessa regional TB dispensary was conducted in 2012 with consideration of CYP2C19 genotype. At the beginning of the treatment the processes of disintegration were 3 times less in patients with *1/*1 genotype than in patients with *1/*2 genotype (10.3% versus 31.8%; P<0.05; χ2=5.40), and 6.5 times less than in individuals with *2/*2 genotype (10.3% versus 66.7%; P<0.05; χ2=7.94). At the end of the in-patient treatment the signs of pulmonary destruction were 6.5 times more common in individuals with *1/*1 genotype than in individuals with *1/*2 genotype (29.3 versus 4.5; Р<0.05; χ2=5.61). The signs of resorption and consolidation in the pulmonary tissue were observed in 74.1% of the patients with *1/*1 genotype, 95.5% of the patients with *1/*2 genotype and 66.7% of the patients with *2/*2 genotype. There were no significant differences between groups in relation to duration of the treatment and development of drug-resistance

Critical parameters of N-vector spin models on 3d lattices from high temperature series extended to order beta^{21}

High temperature expansions for the free energy, the susceptibility and the second correlation moment of the classical N-vector model [also denoted as the O(N) symmetric classical spin Heisenberg model or as the lattice O(N) nonlinear sigma model] have been extended to order beta^{21} on the simple cubic and the body centered cubic lattices, for arbitrary N. The series for the second field derivative of the susceptibility has been extended to order beta^{17}. An analysis of the newly computed series yields updated estimates of the model's critical parameters in good agreement with present renormalization group estimates.Comment: 3 pages, Latex,(fleqn.sty, espcrc2.sty) no figures, contribution to Lattice'97 to appear in Nucl. Phys. Proc. Supp

An impact of CYP3A4 *1B polymorphism on rifampicin metabolism

Until now, the enzyme systems responsible for biotransformation of the antituberculous drug rifampicin remain unknown. The aim of research was an investigation of the candidate enzymes involved in the biotransformation of rifampicin using the computer system PASS and an experimental study concerning the effect of the polymorphism of the biotransformation gene CYP3A4 *1B on the level of rifampicin in the blood of patients with pulmonary tuberculosis (РTB).The probability (Pa) of certain pharmacological activity and the effect on putative enzyme systems of the human body of rifampicin has been calculated by the PASS method. Polymerase chain reaction revealed the polymorphism of the CYP3A4 *1B gene among healthy volunteers as well as patients with РTB.With a high degree of probability, according to PASS calculations, it was predicted that rifampicin undergo metabolism with the CYP3A4 enzyme - probability (Ra) were 0.891. According to the genotype CYP3A4 *1B, 95.3% of the healthy donors carried a homozygous wild-type gene (i.e., had high enzymatic activity) - AA genotype; the rest 4.7% - were carriers of the heterozygous AG genotype (moderate enzyme activity).The polymorphism of CYP3A4 *1B genotypes and alleles in the south-west of Ukraine was close to the results obtained in European countries. 91.4% and 8.6% of the patients with РTB had AA and AG genotype, correspondently. Thus, among the patients with РTB, the AG genotype was more often observed than among healthy volunteers. There was no significant difference in rifampicin concentration among РTB-patients concerning CYP3A4 * 1B polymorphism

High magnetomechanical coupling on magnetic microwire for sensors with biological applications

In this letter, we report the experimental work carried out to study and improve the magnetomechanical coupling on magnetic microwires. A good magnetoelastic response, with magnetoelastic coupling factor k=0.43, has been obtained by control of both wire diameter and sample microstructure. The study addresses two following aspects: (a) analysis of the suitability of amorphous magnetoelastic microwire as promising tiny sensor element to be used as wireless biosensors as well as environment detectors. In particular, its potential as liquids viscosity sensor has been confirmed. (b) Study, as the first time in this kind of magnetic microwire, of the effects of nanocrystallization on the sample magnetoelastic behavior

Critical behavior of frustrated systems: Monte Carlo simulations versus Renormalization Group

We study the critical behavior of frustrated systems by means of Pade-Borel resummed three-loop renormalization-group expansions and numerical Monte Carlo simulations. Amazingly, for six-component spins where the transition is second order, both approaches disagree. This unusual situation is analyzed both from the point of view of the convergence of the resummed series and from the possible relevance of non perturbative effects.Comment: RevTex, 10 pages, 3 Postscript figure

A characterization of those automata that structurally generate finite groups

Antonenko and Russyev independently have shown that any Mealy automaton with no cycles with exit--that is, where every cycle in the underlying directed graph is a sink component--generates a fi- nite (semi)group, regardless of the choice of the production functions. Antonenko has proved that this constitutes a characterization in the non-invertible case and asked for the invertible case, which is proved in this paper